With the introduction of small diameter particles (< 2 µm), HPLC instruments needed to be improved to provide the potential benefits offered by these columns. A number of Ultra-High Performance Liquid Chromatography (UHPLC) systems are now available from HPLC manufacturers. UHPLC systems utilize high-pressure pumps and low extra-column dispersion by minimizing volumes of connecting tubing, detection flow cells and employing high-speed data acquisition units.
UHPLC is the generic industry term, however UPLC (Ultra Performance Liquid Chromatography) is a trademarked name, commonly used when referring to this subject. Both are the same technique and both are a specialized subset of HPLC.
The introduction of the sub-2 µm immobilized chiral columns by Daicel Corporation will allow an increase in resolution and speed for UHPLC chiral chromatography.