21. After a long period of continuous analysis, my chiral column has begun exhibiting a loss of selectivity, peak tailing, and/or peak splitting. What can I do to restore my column’s original performance?

Although quite rare, it is not unknown for polysaccharide-based chiral columns, especially after repeated use of additives or aggressive mobile phase conditions, to experience some loss of performance. Fortunately these effects can be mitigated by utilizing a regeneration procedure. These regeneration procedures utilize extended range solvents like DCM, DMF, or EtOAc to help restore the columns original performance. It is important to note these procedures can only be utilized with the immobilised CHIRALPAK^® chiral columns, and will result in irreversible damage to any coated CHIRALPAK^® or coated CHIRALCEL^® column.
For a detailed regeneration procedure for all sub-2 µm, 3 µm, and 5 µm immobilised CHIRALPAK^® analytical and preparative columns, click here.