SFC has become a popular technique for preparative chromatography. Chiral Technologies has been at the forefront of the technology establishing separation services in 1997. Chiral Technologies has also developed semi-preparative chiral columns specifically designed for SFC applications.
SFC has two main advantages for a number of preparative separations. One is speed: a higher production rate can be obtained since the mobile phase viscosity is very low and fast, efficient separations can be achieved. The other advantage is the small volume of organic solvent used: between 10% and 20% of that needed for a HPLC separation. This not only decreases the total solvent consumption used for the separation, but also makes it easier and faster to recover the products from the small modifier volumes remaining after condensation from the CO2.