Technical Library

Chiral Technologies’ stationary phases are applied to enantioseparations of a great variety of chiral compounds. Technical Library contains Publications in a number of scientific journals.  Publications are grouped by use of specific stationary phases. Technical Library also contains Application Notes and Presentations given at conferences and trade shows.








Polysaccharide Chiral Stationary Phases

Application of Polysaccharide-Based Chiral HPLC Columns for Separation of Nonenantiomeric Isomeric Mixtures of Organometallic Compounds, M. Ogasawara, Y. Enomoto, M. Uryu, X. Yang, A. Kataoka, A. Ohnishi, Organometallics, 38, (2019), 512-518

Simultaneous Separation of Triacylglycerol Enantiomers and Positional Isomers by Chiral High Performance Liquid Chromatography Coupled with Mass Spectrometry, T. Nagai, T. Kinoshita, E. Kasamatsu, K. Yoshinaga, H. Mizobe, A. Yoshida, Y. Itabashi, N. Gotoh, J. Oleo Sci., 68, (2019), 1019-1026

Development and Validation of Stereo Selective Method for the Separation of Razoxane Enantiomers in Hydrophilic Interaction Chromatography, Ch. Thirupathi, K. Nagesh Kumar, G. Srinivasu, Ch. Lakshmi Narayana, Ch. Parameswara Murthy, JCS, 56, (2018), 147-153

Enantioselective separation of racemates using CHIRALPAK IG amylose-based chiral stationary phase under normal standard, non-standard and reversed phase high performance liquid chromatography, A. Ghanem, C. Wang, J. Chromatogr. A, 1532, (2018), 89-97

Enantioseparation of Chiral Sulfoxides on Amylose‑Based Columns: Comparison of Normal Phase Liquid Chromatography and Supercritical Fluid Chromatography, N. Kolderová, T. Neveselý, J. Šturala, M.Kuchař, R. Holakovský, M. Kohout, Chromatographia, 80, issue #4, (2017), 547-557

Simultaneous enantioselective determination of 22 chiral pesticides in fruits and vegetables using chiral liquid chromatography coupled with tandem mass spectrometry P. Zhao, Z. Wang, X. Gao, X. Guo, L. Zhao, Food Chemistry, 277, (2019), 298-306

Enantiomeric separation and simulation study of eight anticholinergic drugs on an immobilized polysaccharide-based chiral stationary phase by HPLC, M. Li, B. Zhang, J. Yu, J. Wang, X. Guo, New J. Chem 42, (2018), 11724-11731

Multi-residue enantiomeric analysis of 18 chiral pesticides in water, soil and river sedi-ment using magnetic solid-phase extraction based on amino modified multiwalled carbon nanotubes and chiral liquid chromatography coupled with tandem mass spectrometry, P. Zhao, et al., J. Chromatogr. A (2018)

The sodium salt of the enantiomers of ricobendazole: Preparation, solubility and chiroptial properties, R. Cirilli, P. Guglielmi, F. Romana Formica, A. Casulli, S. Carradori, J. Pharm. Biochem. Anal., 139, (2017) 1-7

Development of an HPLC method for the simultaneous determination of chiral impurities and assay of (S)-Clopidogrel using a cellulose-based chiral stationary phase in methanol/water mode, R. Ferretti, L. Zanitti, R. Cirilli, J. Sep. Sci., (2017)

A chromatographic study on the exceptional chiral recognition of 2-(benzylsulfinyl) benzamide by an immobilized-type chiral stationary phase based on cellulose tris(3,5-dichlorophenylcarbamate), S. Carradori, D. Secci, C. Faggi, R. Cirilli, J. Chromatogr. A, 1531, (2018), 151-156

Achiral Molecular Recognition of Aromatic Position Isomers by Polysaccharide-Based CSPs in Relation to Chiral Recognition, T. Shibata, S. Shinkura, A. Ohnishi, K. Ueda, Molecules, 22, (2017), 38

On-chip integration of organic synthesis and HPLC/MS analysis for monitoring stereoselective transformations at the micro-scale,  

Enantioselective reaction monitoring utilizing two-dimensional heart-cut liquid chromatography on an integrated microfluidic chip, C. Lotter, E. Poehler, J. Heiland, L. Mauritz, D. Belder, Lab Chip, 16, (2016), 4648–4652

Bioanalytical method for the simultaneous determination of d- and l-serine in human plasma by LC/MS/MS, H. Sugimoto, M. Kakehi, F. Jinno, Anal. Biochem.,487 (2015) 38-44

Multigram Synthesis and in Vivo Efficacy Studies of a Novel Multitarget Anti-Alzheimer’s Compound,
I. Sola, E. Viayna, T. Gomez, C. Galdeano, M. Cassina, P. Camps, M. Romeo, L. Diomede,
M. Salmona, P. Franco, M. Schaeffer, D. Colantuono, D. Robin, D. Brunner, N. Taub, B. Hutter-Paier, D. Muñoz-Torrero, Molecules, 20 (2015) 4492-4515

On the method development of immobilized polysaccharide chiral stationary phases in supercritical fluid chromatography using an extended range of modifiers, J. Lee, J.T. Lee, W.L. Watts, J. Barendt, T.Q. Yan, Y. Huang, F. Riley, M. Hardink, J. Bradow, P. Franco, J. Chromatogr. A (2014)

Polysaccharide Derivatives for Chromatographic Separation of Enantiomers, Y. Okamoto, E. Yashima, Angew. Chem. Int. Ed., 37 (1998) 1020–1043

Enantioselective chromatography as a powerful alternative for the preparation of drug enantiomers, E. R. Francotte, J. Chromatogr. A, 906 (2001) 379-397

Reversed-phase liquid chromatographic separation of enantiomers on polysaccharide type chiral stationary phases, K. Tachibana, A. Ohnishi, J. Chromatogr. A, 906 (2001) 127-154

Polysaccharide-based chiral stationary phases for high-performance liquid chromatographic enantioseparation, E. Yashima, J. Chromatogr. A, 906 (2001) 105-125

Chromatography as a Separation Tool for the Preparative Resolution of Racemic Compounds,  E. R. Francotte, ChemInform, 28 (1997)

Solvent versatility of immobilized 3,5-dimethylphenylcarbamate of amylose in enantiomeric separations by HPLC, T. Zhang, C. Kientzy, P. Franco, A. Ohnishi, Y. Kagamihara, H. Kurosawa, J. Chromatogr. A, 1075 (2005) 65-75

Cellulose tris(3,5-dichlorophenylcarbamate) immobilised on silica: A novel chiral stationary phase for resolution of enantiomers, T. Zhang, D. Nguyen, P. Franco, Y. Isobe, T. Michishita, T. Murakami, J. Pharm. Biochem. Anal., 46 (2008) 882-891

Cellulose 3,5-dimethylphenylcarbamate immobilized on silica: A new chiral stationary phase for the analysis of enantiomers, T. Zhang, D. Nguyen, P. Franco, T. Murakami, A. Ohnishi, H. Kurosawa, Anal. Chim. Acta, 557 (2006) 221-228

Optimization of the chiral separation of a Ca-sensitizing drug on an immobilized polysaccharide-based chiral stationary phase: Case study with a preparative perspective, T. Zhang, M. Schaeffer, P. Franco, J. Chromatogr. A, 1083 (2005) 96-101

Enantiomer resolution screening strategy using multiple immobilised polysaccharide-based chiral stationary phases, T. Zhang, D. Nguyen, P. Franco, J. Chromatogr. A, 1191 (2008) 214-222

Complementary enantiorecognition patterns and specific method optimization aspects on immobilized polysaccharide-derived chiral stationary phases, T. Zhang, P. Franco, D. Nguyen. R. Hamasaki, S. Miyamoto, A. Ohnishi. T. Murakamo, J. Chromatogr. A, 1269 (2012) 178–188

Reversed-phase screening strategies for liquid chromatography on polysaccharide-derived chiral stationary phases, T. Zhang, D. Nguyen, P. Franco, J. Chromatogr. A, 1217 (2010) 1048–1055

Practical access to the proline analogs (S,S,S)- and (R,R,R)-2-ethyloctahydroindole-carboxylic acids by HPLC enantioseparation, F. J. Sayago, M. J. Pueyo, M. I. Calaza, A. I. Jimenez. C. Cativiela, Chirality, 23, (2011) 507–513

Organocatalytic Aryl-Aryl Bond-Formation: An Atroposelective [3,3]-Rearrangement Approach to BINAM Derivatives, G-Q. Li, H. Gao, C. Keene, M. Devonas, D. H. Ess, L. Kurti, Am. Chem. Soc., 135 (2013) 7414–7417

Common approaches for efficient method development with immobilised polysaccharide-derived chiral stationary phases, P. Franco, T. Zhang, J. Chromatogr. B, 875 (2008) 48

Novel stereoselective synthesis and chromatographic evaluation of E-guggulsterone, A. Gioiello, R. Sardella, E. Rosatello, B. Sadeghpour, B. Natalini, R. Pellicciari, Steroids, 77 (2012) 250–254

Preparative enantioseparations using supercritical fluid chromatography, L. Miller, J. Chromatogr. A, 1250 (2012) 250-255

Evaluation of non-traditional modifiers for analytical and preparative enantioseparations using supercritical fluid chromatography, L. Miller, J. Chromatogr. A, 1256 (2012) 261-266

High-performance liquid chromatographic separation of enantiomers and diastereomers of 2-methylcyclohexanone thiosemicarbazone, and determination of absolute configuration and configurational stability, R. Cirilli, R. Ferretti, F. La Torre, D. Secci, A. Bolasco, S. Carradoni, M. Pierini, J. Chromatogr. A, 1172 (2007) 160-169

High-performance liquid chromatography enantioseparation of proton pump inhibitors using the immobilized amylose-based CHIRALPAK IA chiral stationary phase in normal-phase, polar organic and reversed-phase conditions, R. Cirilli, R. Ferretti, B. Gallinella, E. De Santis, L. Zanitti, F. La Torre, J. Chromatogr. A, 1177 (2008) 105-113

Analytical and semipreparative high performance liquid chromatography enantioseparation of new substituted 1-thiocarbamoyl-3,5-diaryl-4,5-dihydro-(1H)-pyrazoles on polysaccharide-based chiral stationary phases in normal-phase, polar organic and reversed-phase conditions, R. Cirilli, A. Simonelli, R. Ferretti, A. Bolasco, P. Chimenti, D. Secci, E. Maccioni, F. La Torre, J. Chromatogr. A, 1101 (2006) 198-203

Direct HPLC enantioseparation of chiral aptazepine derivatives on coated and immobilized polysaccharide-based chiral stationary phases, R. Cirilli, V. Orlando, R. Ferretti, L. Turchetto, R. Silvestri, G. De Martino, F. La Torre, Chirality 18, (2006) 621-632

Development and validation of an enantioselective and chemoselective HPLC method using a CHIRALPAK IA column to simultaneously quantify (R)-(+)- and (S)-(−)-lansoprazole enantiomers and related impurities, R. Cirilli, R. Ferretti, B. Gallinella, L. Turchetto, L. Zanitti, F. La Torre, J. Pharm. Biochem. Anal., 50, (2009) 9-14

Direct high-performance liquid chromatography enantioseparation of terazosin on an immobilised polysaccharide-based chiral stationary phase under polar organic and reversed-phase conditions, R. Ferretti, B. Gallinella, F. La Torre, L. Zanitti, L. Turchetto, A. Mosca, R. Cerilli, J. Chromatogr. A, 1216 (2009) 5385-5390

Synthesis and chromatographic enantioresolution of anti-HIV quinolone derivatives, B. Natalini, R. Sardella, S. Massari, F. Ianni, O. Tabarrini, V. Cecchetti, Talanta, 85, (2011) 1392–1397

Evaluation of non-conventional polar modifiers on immobilized chiral stationary phases for improved resolution of enantiomers by supercritical fluid chromatography, J. O. DaSilva, B. Coes, L. Frey, I. Mergelsberg, R. McClain, L. Nogle, C. J. Welch, J. Chromatogr. A, 1328 (2014) 98-103

T. Zhang, P. Franco, Analytical and preparative potential of immobilized polysaccharide-derived chiral stationary phases, in Chiral Separation Techniques – A Practical Approach, third ed., G. Subramanian (Editor), Wiley-VCH, Weinheim, Germany, 2006, 99-134

G.B. Cox, Introduction to preparative chromatography, in Preparative Enantioselective Chromatography, G.B. Cox (Editor), Blackwell Publishing Ltd, Oxford, UK, 2005

P. Franco, T. Zhang, Common screening approaches for efficient analytical method development in LC and SFC on columns packed with immobilized polysaccharide stationary phases, in Chiral Separations, Methods and Protocols, second ed., G. Scriba (Editor), Humana Press, 2013, 113-126

Anion-Exchange Chiral Stationary Phases

Ultratrace analysis of enantiomeric impurities in proteinogenic N-Fmoc-amino acid samples on Cinchona alkaloid-based chiral stationary phases, A. Péter, N. Grecsó, G. Tóth, F. Fülöp, W. Lindner, I. Ilisz, Israel Journal of Chemistry 56 (2016) 1042-1051

Improved chromatographic diastereoresolution of cyclopropyl dafachronic acid derivatives using chiral anion exchangers, F. Ianni, L. Pucciarini, A. Carotti, A. Gioiello, R. Galarini, S. Natalini, R. Sardella, W. Lindner, B. Natalini, J. Chromatogr. A., 1557, (2018) 20–27

Chiral separation of disease biomarkers with 2-hydroxycarboxylic acid structure, C. Calderón, C. Santi, M. Lämmerhofer, J. Sep. Sci., (2017), 1-8

Quinine and quinidine derivatives as chiral selectors I. Brush type chiral stationary phases for HPLC based on cinchonan carbamates and their application as chiral anion exchangers, M. Lämmerhofer, W. Lindner, J. Chromatogr. A, 741 (1996) 33-48

Chiral anion exchange-type stationary phases based on cinchonan alkaloids. An effective tool for the separation of enantiomers of chiral acids, M. Lämmerhofer, N. M. Maier, W. Lindner, ChemInform, 29 (1998)

Quinine- versus carbamoylated quinine-based chiral anion exchangers: A comparison regarding enantioselectivity for N-protected amino acids and other chiral acids, A. Mandl, L. Nicoletti, M. Lämmerhofer, W. Lindner, J. Chromatogr. A, 858 (1996) 1-11

Enantioseparation of various amino acid derivatives on a quinine based chiral anion-exchange selector at variable temperature conditions. Influence of structural parameters of the analytes on the apparent retention and enantioseparation characteristics, W. R. Oberleitner, N. M. Maier, W. Lindner, J. Chromatogr. A, 960 (2002) 97-108

Use of evaporative light scattering detector in the detection and quantification of enantiomeric mixtures by HPLC, T. Zhang, D. Nguyen, P. Franco, J. Sep. Sci., 29 (2006) 1517-1524

Kinetic resolution of glyceraldehyde using an aldehyde dehydrogenase from Deinococcus geothermalis DSM 11300 combined with electrochemical cofactor recycling, H. Wulf, M. Perzborn, G. Sievers, F. Scholz, U.T. Bornscheuer, Journal of Molecular Catalysis B: Enzymatic, 74 (2012) 144-150

Enantiomer and topoisomer separation of acidic compounds on anion-exchanger chiral stationary phases by HPLC and SFC, P. Franco, T. Zhang, A. Gargano, M. Mahut, M. Lämmerhofer, W. Lindner, LCGC Europe, 25 (2012) 600-611

Potential of chiral anion-exchangers operated in various subcritical fluid chromatography modes for resolution of chiral acids, R. Pell, W. Lindner, J. Chromatogr. A, 1245 (2012) 175-182

Phosphopeptidomimetic substance libraries from multicomponent reaction: Enantioseparation on quinidine carbamate stationary phase, A. F. G. Gargano, W. Lindner, M. Lämmerhofer, J. Chromatogr. A, 1310 (2013) 56– 65

Simultaneous diastereo- and enantioseparation of farnesoid X receptor (FXR) agonists with a quinine carbamate-based chiral stationary phase, R. Sardella, M. Marinozzi, F. Iammi, A. Lisanti, B. Natalini, Anal. Bioanal. Chem., 405 (2013) 847-862

2-Acyl-dimedones as UV-active protective agents for chiral amino acids: enantiomer separations of the derivatives on chiral anion exchangers, S. Wernisch, F. Bisi, A. S. Cazzato, M. Kohout, W. Lindner, Anal. Bioanal. Chem., 405 (2013) 8011–8026

Zwitterionic Chiral Stationary Phases

Effects of N-methylation and amidination of cyclic ß-amino acids on enantioselectivity and retention characteristics using Cinchona alkaloid- and sulfonic acid-based chiral zwitterionic stationary phases, T. Orosz, E. Forró, F. Fülöp, W. Lindner, I. Ilisz, A. Péter, J. Chromatogr. A, 1535 (2018) 72-79

Electrostatic attraction-repulsion model with Cinchona alkaloid-based zwitterionic chiral stationary phases exemplified for zwitterionic analytes, V. Mimini, F. Ianni, F. Marini, H. Hettegger, R. Sardella, W. Lindner, Analytica Chimica Acta, (2019)

A chiral unified chromatography-mass spectrometry method to analyze free amino acids, A. Raimbault, M. Dorebska, C. West, Analytical and Bioanalytical Chemistry, (2019), 1-9

Stereoselective separation of underivatized and 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate derivatized amino acids using zwitterionic quinine and quinidine type stationary phases by liquid chromatography–High resolution mass spectrometry, J. Horak, M. Lämmerhofer, J. Chromatogr. A, (2019)

Direct Separation of Pregabalin Enantiomers Using a Zwitterionic Chiral Selector by High Performance Liquid Chromatography Coupled to Mass Spectrometry and Ultraviolet DetectionL. Narayana Chennuru, T. ChoppariR. Prasad Nandula, T. Zhang and P. Franco, Molecules, 21(11), 2016, 1578

Exploring the enantiorecognition mechanism of Cinchona alkaloid-based zwitterionic chiral stationary phases and the basic trans-paroxetine enantiomers, R. Sardella, A. Macchiarulo, F. Urbinati, F. Ianni, A. Carotti, M. Kohout, W. Lindner, A. Peter, I. Ilisz, J. Sep. Sci., (2017)

Liquid  chromatographic  enantioseparation  of  limonene-based  carbocyclic  β-amino acids on zwitterionic Cinchona alkaloid based chiral stationary phases, G. Lajkó, T. Orosz, I. Ugrai, Z. Szakonyi. F. Fülöp, W. Lindner, A. Péter, I. Ilisz, J. Sep. Sci., 40, (2017), 3196-3204

A comparative study for the liquid chromatographic enantioseparation of cyclic β-amino acids and cyclic β-aminohydroxamic acids on Cinchona alkaloid-based zwitterionic chiral stationary phases, A. Bajtai, B. Fekete, M. Palkó, F. Fülöp, W. Lindner, M. Kohout, I. Ilisz, A. Péter, J. Sep. Sci., (2018), 1216-1223

Liquid and subcritical fluid chromatographic enantioseparation of N-Fmoc proteinogenic amino acids on Quinidine-based zwitterionic and anion-exchanger type chiral stationary phases. A comparative study, G. LajkóN. Grecsó, G.  TóthF. Fülöp, W. LindnerA. Péter, I. Ilisz, Chirality, 29 (2017), 225-238

Peptide Analysis: Zwitterionic Chiral Ion-Exchangers as Complementary Option to HILIC and to Reversed-Phase Chromatography, T. Zhang, E. Holder, P. Franco, M. Lämmerhofer, A. Sievers-Engler, H. Gerhardt, H. Gross, W. Lindner, LCGC Europe, 29 (2016) 112-128

Method development for the determination of d- and l-isomers of leucine in human plasma by high-performance liquid chromatography tandem mass spectrometry and its application to animal plasma samples, H. Sugimoto, M. Kakehi, F. Jinno, Anal. Bioanal. Chem., 407 (2015) 7889-7898

Unusual temperature-induced retention behavior of constrained β-amino acid enantiomers on the zwitterionic chiral stationary phases ZWIX(+) and ZWIX(-), I. Ilisz, Z. Pataj, Z. Gecse, Z. Szakonyi, F. Fülöp, W. Lindner, A. Péter, Chirality, 26 (2014) 385-93

Structural and temperature effects on enantiomer separations of bicyclo[2.2.2]octane-based 3-amino-2-carboxylic acids on cinchona alkaloid-based zwitterionic chiral stationary phases, I. Ilisz, N. Grecsó, M. Palkó, F. Fülöp, W. Lindner, A. Péter, J. Pharm. Biomed. Anal., 98 (2014) 130-139

Effect of mobile phase composition on the liquid chromatographic enantioseparation of bulky monoterpene-based β-amino acids by applying chiral stationary phases based on Cinchona alkaloid, Z. Pataj, I. Ilisz, Z. Gecse, Z. Szakonyi, F. Fülöp, W. Lindner, A. Péter, J. Sep. Sci., 37 (2014) 1075-1082

Liquid chromatographic enantiomer separation with special focus on zwitterionic chiral ion-exchangers, M. Lämmerhofer, Anal. Bioanal. Chem., 406 (2014) 6095-6103

Simultaneous quantification of mefloquine (+)- and (-)- enantiomers and the carboxy metabolite in dried blood spots by liquid chromatography/tandem mass spectrometry, M. C.K. Geditz, W. Lindner, M. Lämmerhofer, G. Heinkele, R. Kerb, M. Ramharter, M. Schwab, U. Hofmann, J. Chromatogr. B, 968 (2014) 32–39

Direct high-performance liquid chromatographic enantioseparation of secondary amino acids on Cinchona alkaloid-based chiral zwitterionic stationary phases. Unusual temperature behavior, I. Ilisz, Z. Gecsea, Z. Pataja, F. Fülöpb, G. Tóthc, W. Lindner, A. Péter, J. Chromatogr. A, 1363 (2014) 169-177

Direct enantioseparation of underivatized aliphatic 3-hydroxyalkanoic acids with a quinine-based zwitterionic chiral stationary phase, F. Ianni, Z. Pataj, H. Gross, R. Sardella, B. Natalini, W. Lindner, M. Lämmerhofer, J. Chromatogr. A, 1363 (2014) 101-108

Zwitterionic chiral stationary phases based in cinchona and chiral sulfonic acids for the direct stereoselective separation of amino acids and other amphoteric compounds, T. Zhang, E. Holder, P. Franco, W. Lindner, J. Sep. Sci., 37 (2014) 1237-1247

Method development and optimization on cinchona and chiral sulfonic acid-based zwitterionic stationary phases for enantiomer separations of free amino acids by high-performance liquid chromatography, T. Zhang, E. Holder, P. Franco, W. Lindner, J. Chromatogr. A, 1363 (2014) 191-199

Direct high-performance liquid chromatographic enantioseparation of free α-, β- and γ-aminophosphonic acids employing cinchona-based chiral zwitterionic ion exchangers, A. F. G. Gargano, M. Kohout, P. Macikova, M. Lämmerhofer, W. Lindner, Anal. Bioanal. Chem., 405 (2013) 8027–8038

Enantioselective recognition at mesoporous chiral metal surfaces, C. Wattanakit, Y. Bon Saint Côme, V. Lapeyre, P. A. Bopp, M. Heim, S. Yadnum, S. Nokbin, C. Warakulwit, J. Limtrakul, A. Kuhn, Nat. Commun., 5, 3325 (2014)

Stationary phase-related Investigations of quinine-based zwitterionic chiral stationary phases operated in anion-, cation-, and zwitterion-exchange modes, C. Hoffmann, R. Reischl, N. M. Maier, M. Lämmerhofer, W. Lindner, J. Chromatogr. A, 1216 (2009) 1147-1156

Investigations of mobile phase contributions to enantioselective anion and zwitterion exchange modes on quinine-based zwitterionic chiral stationary phases, C. Hoffmann, R. Reischl, N. M. Maier, M. Lämmerhofer, W. Lindner. J. Chromatogr. A, 1216 (2009) 1157-1166

Synergistic effects on enantioselectivity of novel zwitterionic chiral stationary phases for separations of chiral acids, bases, and amino acids by HPLC, C. Hoffmann, R. Pell, M. Lämmerhofer, W. Lindner, Anal. Chem., 80 (2008) 8780-8789

Increments to chiral recognition facilitating enantiomer separations of chiral acids, bases and ampholytes using cinchona-based zwitterion exchanger chiral stationary phases, S. Wernisch, R. Pell, W. Lindner, J. Sep. Sci., 3 (2012) 1560-1572

Diastereoselective discrimination of lysine-alanine peptides by zwitterionic cinchona alkaloid-based chiral selectors using electrospray ionization mass spectrometry, J. M. Bobbitt, L. Li, D. D. Carlton, M. Yasin, S. Bhawal, F. W. Foss, S. Wernisch, R. Pell, W. Lindner, K. A. Schug, J. Chromatogr. A, 1269 (2012) 308-315

Versatility of cinchona-based zwitterionic chiral stationary phases: Enantiomer and diastereomer separations of non-protected oligopeptides utilizing a multi-modal chiral recognition mechanism, S. Wernisch, W. Lindner, J. Chromatogr. A, 1269 (2012) 297-307

Mechanistic investigations of cinchona alkaloid-based zwitterionic chiral stationary phases, R. Pell, S. Sic, W. Lindner, J. Chromatogr. A, 1269 (2012) 287-296

Enantioseparation of 6-aminoquinolyl-n-hydroxysuccinimidyl carbamate tagged amino acids and other zwitterionic compounds on cinchona-based chiral stationary phases, R. Hellinger, J. Horak, W. Lindner, Anal. Bioanal. Chem., 405 (2013) 8105-8120

Application of cinchona-sulfonate-based chiral zwitterionic ion exchangers for the separation of proline-containing dipeptide rotamers and determination of on-column isomerization parameters from dynamic elution profiles, S. Wernisch, O. Trapp, W. Lindner, Anal.Chim Acta, 795 (2013) 88-98

Enantioseparation of β2-amino acids on cinchona alkaloid-based zwitterionic chiral stationary phases. Structural and temperature effects, I. Ilisz, N. Grecsó, A. Aranyi, P. Suchotin, D. Tymecka, B. Wilenska, A. Misicka, F. Fülöp, W. Lindner, A. Péter, J. Chromatogr. A, 1334 (2014) 44-54

Automated and simultaneous two-dimensional micro-high-performance liquid chromatographic determination of proline and hydroxyproline enantiomers in mammals, Y. Tojo, K. Hamase, M. Nakata, A. Morikawa, M. Mita, Y. Ashida, W. Lindner, K. Zaitsu, J. Chromatogr. B, 875 (2008) 174-179

Simultaneous determination of d-aspartic acid and d-glutamic acid in rat tissues and physiological fluids using a multi-loop two-dimensional HPLC procedure, H. Han, Y. Miyoshi, K. Ueno, C. Okamura, Y. Tojo, M. Mita, W. Lindner, K. Zaitsu, K. Hamase, J. Chromatogr. B, 879 (2011) 3196-3202

Enantioselective two-dimensional high-performance liquid chromatographic determination of n-methyl-d-aspartic acid and its analogues in mammals and bivalves, R. Koga, Y. Miyoshi, E. Negishi, T. Kaneko, M. Mita, W. Lindner, K. Hamase, J. Chromatogr. A, 1269 (2012) 255-261

Protein-Based Chiral Stationary Phases

New approaches of LC-MS compatible method development on α1-acid glycoprotein-based stationary phase for resolution of enantiomers by HPLC, T. Michishita, P. Franco, T. Zhang, J. Sep. Sci., 3627 (2010) 23-24

Trace analysis of fluoxetine and its metabolite norfluoxetine. Part I: Development of a chiral liquid chromatography – tandem mass spectrometry method for wastewater samples, V. K. H. Barclay, N. L. Tyrefors, I. M. Johansson, C. E. Pettersson, J. Chromatogr. A, 1218 (2011) 5587-5596

Enantiomeric analysis of drugs of abuse in wastewater by chiral liquid chromatography coupled with tandem mass spectrometry, B. Kasprzyk-Hordern, V. V. R. Kondakal, D. R. Baker, J. Chromatogr. A, 1217 (2010) 4575-4586

HPLC/SFC columns (achiral)

Chaotropic Effects in Sub/Supercritical Fluid Chromatography via Ammonium Hydroxide in Water-Rich Modifiers: Enabling Separation of Peptides and Highly Polar Pharmaceuticals at the Preparative
Scale, J. Liu, A. Makarov, R. Bennett, I. Haidar Ahmad, J. DaSilva, M. Reibarkh, I. Mangion, B. Mann, E. Regalado, Analytical Chemistry, (2019)

State-of-the-art enantioseparations of natural and unnatural amino acids by high-performance liquid chromatography, I. Ilisz, A. Péter, W. Lindner, Trends in Analytical Chemistry, 81 (2016), 11-22

On-line coupling of achiral Reversed Phase Liquid Chromatography and chiral Supercritical Fluid Chromatography for the analysis of pharmaceutical compounds, M. Iguiniz, E. Corbel, N. Roques, S. Heinisch, J. Pharm. Biomed. Anal, 159, (2018), 237-244

Characterization of Novel Polymer-Based Pyridine Stationary Phases for Supercritical Fluid Chromatography, C. West, E. Lemasson, K. Nagai, T. Shibata, P. Franco, S. Bertin, P. Hennig, E. Lesellier, Chromatographia, (2018), 1-10

Poly(4-vinylpyridine) based novel stationary phase investigated under supercritical fluid chromatography conditions, K. Nagai, T. Shibata, S. Shinkura, A. Ohnishi, J. Chromatogr. A., (2018)

Polymeric stationary phases based on poly(butylene terephthalate) and poly(4-vinylpirydine) in the analysis of polyphenols using supercritical fluid chromatography. Application to bee pollen, L. Toribio, S. Arranz, A. Ares,  J. Bernal, J. Chromatogr. A, (2018)

Poly(butylene terephthalate) based novel achiral stationary phase investigated under supercritical fluid   chromatography conditions, K. Nagai, T. Shibata, S. Shinkura, A. Ohnishi, J. Sep. Sci., (2018)

Application Notes

Achiral Supercritical Fluid Chromatography – Separation of Hydrobenzoin

Separation of the Enantiomers of (+/-) Delta 8-THC and (+/-) Delta 9-THC

Separation of Naproxen using the new immobilized column – CHIRALPAK IH

Introducing the First sub-2 Micron Chiral Columns From Daicel

Separation of Methyclothiazide Using New Immobilized Column – CHIRALPAK IG

Separation of Free Amino Acids and Primary Amines Using Daicel Crown Ether Columns: CROWNPAK CR-I(+) and CROWNPAK CR-I(-)

High-Speed SFC Enantiomeric Separation Using the Optimal Daicel SFC Chiral Columns

Separation of enantiomers and conformers of Tofisopam using Daicel immobilized polysaccharide-derived chiral columns using the Agilent 1260 Infinity Analytical SFC System, T. Zhang, N. Nguyen, P. Franco, M. Vollmer, Application Note (Agilent, 2011)

Enantiomer separation of non-steroidal anti-inflammatory drugs, T. Zhang, N. Nguyen, P. Franco, M. Vollmer, Application Note (Agilent, 2011)

Enantiomer separation of acidic compounds using Daicel CHIRALPAK QN-AX and QD-AX columns and the Agilent 1260 Infinity Analytical SFC System, T. Zhang, N. Nguyen, P. Franco, M. Vollmer, Application Note (Agilent, 2011)

Effective Separations of DL-Leu-DL-Val on CHIRALPAK ZWIX(+)and DL-Ala-DL-Leu-Gly using CHIRALPAK ZWIX(-)

CHIRALPAK® IA-3 for Measurement of Atorvastatin Enantiomeric Purity – New Method to Achieve Highly Reliable Results with High-Throughput Analysis

Pressure Stability of Wide-Pore Diameter Chiral Chromatography Columns

Two Novel Polysaccharide-Based Chiral Stationary Phases: CHIRALPAK® AY-H and CHIRALCEL® OZ-H

Reversed-Phase Enantioselective Chromatography with New 3-micron Chiral Stationary Phases

Fast Enantioselective Chromatography with 3-micron Particles

Daicel 3-micron CHIRALPAK® and CHIRALCEL® Analytical Columns

Enantioselective Supercritical Fluid Chromatography using Daicel’s Immobilized Polysaccharide-Based Columns

CHIRALPAK® IC – An Immobilized Polysaccharide Chiral Stationary Phase with a Unique Chiral Selector

Chiral Separations on a New Commercially Available Immobilized Cellulose Column, CHIRALPAK® IB

Empirical Determination of the Operating Conditions for the SMB Separation of 1-phenyl-1-methylsuccinimide Enantiomers

Extending the Range of Solvents for Chiral Analysis Using a New Immobilized Polysaccharide Chiral Stationary Phase, CHIRALPAK® IA

Versatile Sample Diluents for CHIRALPAK® IA Immobilized Chiral Stationary Phase: Dimethylsulfoxide Injection Studies

Enantioselective Reversed-Phase Chromatography with Protein-Based Columns

SMB Process Development and Optimization Using Immobilized Chiral Stationary Phases: Chiral Separation of 1-Methyl-1-Phenylsuccinimide Using a THF Based Mobile Phase


Modern Methods for the Separation of Enantiomers – from Kilos to Tons – Organic Process Research and Development 2014, Orlando, FL

Chiral SFC Method Development of Immobilized Polysaccharide-Derived CSPs Using Non-Conventional Modifiers – HPLC 2014, New Orleans, LA

Multimilligram Stack Injection Chiral Separation of MDMA – HPLC 2014, New Orleans, LA

Advances in Method Development for Preparative Chiral Chromatography – Prep 2014, Boston, MA

Separation of Chiral Pesticides by SFC and HPLC – IUPAC International Congress 2014, San Francisco, CA

Direct Stereo-Selective Separations of Amino Acids and Small Peptides on Cinchona-Derived Zwitterionic Chiral Columns by HPLC – USP Workshop 2014, Synthetic Therapeutic Peptides: Regulations, Standards and Quality, Rockville, MD

On the Method Development of Immobilized Polysaccharide Chiral Stationary Phases in SFC Using Extended Range of Co-Solvents – SFC/Green Chemistry 2013, Boston, MA

Preparative Chiral Chromatography – SPICA 2012, Brussels, Belgium

Chirality in the Natural Sciences – AgroChemical Conference 2012, Cambridge, UK

Direct Stereo-Selective Separations of Free Amino Acids on Quinine- and Quinidine-based Zwitterionic Chiral Stationary Phases by HPLC – HPLC 2012, Anaheim, CA

Chirality. Asymmetric, Enzymatic…Is there a better way? – ChemOutsourcing 2012, Long Branch, NJ

Novel Immobilized Polysaccharide-derived CSPs to Enhance Success Rate in the Resolution of Enantiomers – HPLC 2011, Budapest, Hungary

Supercritical Fluid Chromatography Screening Approach for Chiral Separations – ChromSoc Spring Symposium 2011, Horsham, UK

Enantiomer Separation of Chiral Acids on CHIRALPAK® AX-QN and CHIRALPAK®AX QD – PREP Symposium 2010, Philadelphia, PA

New 3-Micron Polysaccharide-based Chiral Columns for Fast HPLC and SFC – PittCon 2009, Chicago, IL

Determination of Enantiomeric Excess with Evaporative Light Scattering Detectors (ELSD): Why racemic mixtures do not show a 50:50 ratio – Drug Analysis 2006, Namur, Belgium




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